中国寄生虫学与寄生虫病杂志 ›› 1994, Vol. 12 ›› Issue (1): 37-39.

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聚合酶链反应(PCR)技术在恶性疟诊断中的应用

詹斌,张龙兴,王聚君,冯晓平   

  1. 中国预防医学科学院寄生虫病研究所
  • 出版日期:1994-02-28 发布日期:2017-01-06
  • 基金资助:
    国家自然科学青年基金

DIAGNOSIS OF FALCIPARUM MALARIA BY POLYMERASE CHAIN REACTION 

  • Online:1994-02-28 Published:2017-01-06

摘要: 应用聚合酶链反应(PCR)检测云南疟疾病人血样53份,结果均为阳性,可出现一条492bp的特异条带,与镜检符合率达100%;同时检测间日疟血样8份及上海正常人血样12份,均为阴性,表明本法具有高敏感性及特异性。

关键词: 诊断, 恶性疟, 聚合酶链反应

Abstract: Polymerase Chain Reaction(PCR) method using a pair of Plasmodium falciparum specific oligonucleotides as primers was used to detect malaria patient blood samples collected from Yunnan endemic malarious area.The results showed that a DNA fragment of 492 bp was amplified in all 53 microscopically diagnosed P.falciparum samples,but negative in 8 P. vivax samples and 12 normal blood samples.This method seems to have high sensitivity and high specificity.

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