关键词: 恶性疟原虫, N-乙酰葡萄糖胺, 免疫胶金标记

Abstract: In this study p aminopheny1 2 acetamido 2 deoxy β D glucopyranoside was bound covalently to bovine serum albumin(BSA)to form neoglycoprotein(GlcNAc BSA).The antigenicity of BSA and the pyranose structure of the sugar was preserved.By using the neoglycoprotein antibody against BSA and staphylococcal protein A gold(SPA gold), Plasmodium falciparum FCC 1/HN merozoites were immunolabelled.The labelled sample was observed under transmission electron microscope(TEM).The TEM pictures showed that colloidal gold pellets were distributed all over the merozoite surface.This is the first report on the direct experimental evidence of molecular recognition between GlcNAc BSA(or GlcNAc)and Plasmodium falciparum merozoites.But free GlcNAc or even GlcN can inhibit the immunolabeleation.The results support the hypothesis that,besides N acetylneuraminic acid,GlcNAc is involved as another recognized site on GPA for Plasmodium falciparum .Thus we further confirmd that N acetyl of GlcNAc is not necessary for the recognition.