关键词: 猪囊尾蚴, 尿素溶性抗原, 水溶性抗原, SDS-PAGE, ELIB, ELISA

Abstract: Urea-soluble antigens (Ag-u) and water-soluble antigens (Ag-w) of Cysticercus cellu-losae were studied by using ELIB and ELISA, respectively. The results showed that using Ag-u and Ag-w, 9 and 14 bands of polypeptides could be recognized by sera from cysticerco-sis patients; 4 and 10 bands could be recognized by sera from hydatidosis patients; whereas 4 and 8 bands could be recognized by sera from clornochiasis patients.Using ELISA at serum dilutions of 1 : 400, the positive rate of sera against Ag-u and Ag-w was 96. 6% and 86. 6% .respectively in 60 cysticercosis patients; 16. 6% and 46. 6% respectively in 30 hydatidosis patients and negative in 24 clornorchiasis patients and 50 normal healthy persons, indicating that both Ag-u and Ag-w share common antigen components causing cross reaction with the sera from hydatidosis and clonorchiasis patients. However, such components were fewer in Ag-u than in Ag-w. In view of the fact that Ag-u is superior to Ag-w in both sensitivity and specificity, Ag-u might be better used for the diagnosis of cysticercosis.