中国寄生虫学与寄生虫病杂志 ›› 1993, Vol. 11 ›› Issue (1): 38-40.
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姜昌斌,杨惠珍,杨鹤萍,杨杨,徐克继
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摘要： 本研究应用聚丙烯酸胺凝胶电泳及免疫印迹技术,对我们分别引进或从畸胎中分离所得的弓形虫株——RH株、Z-2株、CN株和SH1、2、3、4、5、8株的蛋白质和抗原成份进行分析,结果表明,在蛋白质的主要区带方面,9株弓形虫比较接近,而在部分区带上有所不同;用高滴度的针对RH株的抗弓形虫抗体分析9株弓形虫抗原成份,可见有一些共同的抗原成份。
关键词: 聚丙烯酰胺凝胶电泳, 免疫印迹技术, 弓形虫株
Abstract: The proteins and antigens of nine strains of Toxoplasma gondii were analyzed by SDS-PAGE and immunoblotting. These strains including RH strain, Zs-2 strain, CN strain and SHI, SH2, SH3, SH4, SH5, SH8 strains, were obtained from other laboratories or isolated from deformity fetus in our laboratory. SDS-PAGE analysis revealed that these strains were very close in the major bands of proteins and differences were observed only in part. There were common components in antigens of all strains using IgG antibody prepared from high-titre rabbit antisera raised against RH strain of Toxoplasma.
姜昌斌,杨惠珍,杨鹤萍,杨杨,徐克继. 应用聚丙烯酰胺凝胶电泳及免疫印迹技术分析弓形虫蛋白质与抗原的研究[J]. 中国寄生虫学与寄生虫病杂志, 1993, 11(1): 38-40.
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