中国寄生虫学与寄生虫病杂志 ›› 1992, Vol. 10 ›› Issue (4): 307-310.

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泡球蚴冰冻保存后感染力的初步观察

蒋次鹏   

  1. 兰州医学院包虫病研究室
  • 收稿日期:2017-01-07 修回日期:2017-01-07 出版日期:1992-11-30 发布日期:2017-01-09

PRELIMINARY OBSERVATION ON INFECTIVITY OF ISOLATED ALVEOLAR HYDATID AFTER CRYOPRESERVATION

  • Received:2017-01-07 Revised:2017-01-07 Online:1992-11-30 Published:2017-01-09

摘要: 将泡球蚴保存于-10℃130d后,再移植至小鼠腹腔内,并分别于接种后第67d和225d剖检,均见虫周围有淋巴细胞浸润和纤维组织增生,有的形成淋巴性坏死、坏死腔、钙颗粒,示泡球蚴已丧失活力;而将泡球蚴保存于-30℃200d后,在接种后第157d剖检,可见囊壁角质层、生发膜清晰,囊内有多个原头节及其小钩结构、母囊泡、子囊泡和大小不等的钙颗粒等,表明其有活力和感染力。结果提示冰冻保存离体泡球蚴的活力与温度有关,-30℃比-10℃更有利于泡球蚴的存活。

关键词: 泡球蚴, 多房棘球绦虫, 冰冻保存

Abstract: Alveolar hydatids of Echinococcus multilocularis obtained from the peritoneal cavity of mice were subject to freezing at-10℃ and-30℃ respectively.After 130-200 days' storage,the alveolar hydatids were transferred into the mouse peritoneal cavity for viability and inactivity testing.It was found that the alveolar hydatids stored at-10℃ were more severely damaged than those stored at-30℃.The viability of the isolated alveococcus lasted half a year at-30℃.