关键词: 日本血吸虫, 品系, 酶联免疫电转移印迹

Abstract: Antigens prepared from S.japonicum adult worms of different isolates i.e.Anhui,Hubei,Guangxi,Yunnan and Sichuen by origin were analyzed by enzyme linked immunoe-lectrotransfer blot (EITB) probed with rabbit anti-snail antibody (Figs 1,2).Anti-sera against Oncomelania h,hupensis from Anhui,Hubei,Yunnan and Sichuan localities were prepared separately and used in the tests.The EITB patterns were similar in S.j.isolates of Anhui and Hubei,and it was also the case among S.7.isolates of Yunnan and Sichuan except Yunnan female worms with a marked band of 84 kDa but it was almost invisible in EITB pattern of Sichuan female worms.Like Yunnan isolate,female worms of Guangxi isolate also showed marked 84 kDa bands.The EITB pattern of male worms from Guangxi isolate showed 2 main bands of mw130 kDa against anti-Anhui snail anti-serum,which corresponded with the male worms of Anhui isolate whereas the color of the bands was darker and denser in the former isolates,and these bands can not be seen in the male worms from isolates of Yunnan and Sichuan.Based on the above mentioned results in connection with the information about the susceptibility between different isolates of schistosemes and their snail hosts which we have reported before,some preliminary analysis and discussion were made.