中国寄生虫学与寄生虫病杂志 ›› 1992, Vol. 10 ›› Issue (2): 104-107.

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中国大陆日本血吸虫品系的研究——Ⅷ.感染动物血清免疫学反应

薛海筹,何毅勋,裘丽姝,张永红   

  1. 中国预防医学科学院寄生虫病研究所
  • 出版日期:1992-05-31 发布日期:2017-01-10
  • 基金资助:
    国家自然科学基金

STUDIES ON THE STRAIN DIFFERENCES OF SCHISTOSOMA JAPONICUM IN THE MAINLAND OF CHINA VIII. IMMUNOREACTIONS IN EXPERIMENTAL ANIMALS

  • Online:1992-05-31 Published:2017-01-10

摘要: 本文观察了安徽、湖北、四川、云南、广西5地日本血吸虫感染小鼠、大鼠、金黄仓鼠、兔、恒河猴等5种动物的血清对同源及异源虫卵的环卵沉淀试验(COPT)反应状况以及应用安徽虫卵制备的可溶性抗原进行胶乳凝集试验(LAT)及酶联免疫吸附试验(ELISA)检测同源或异源虫体所致的血吸虫病的抗体情况,未见应用同源虫卵抗原检测感染动物的结果显著地优于异源的。这提示在中国大陆应用异地血吸虫卵抗原进行上述血清免疫学反应检测时似不影响其阳性检出率。

关键词: 日本血吸虫, 环卵沉淀反应, 胶乳凝集试验, 酶联免疫吸附试验

Abstract: Observations on the immunoreactivities toward egg antigens of different experimental animals infected with Schistosoma japonicum were made,which included mouse,rat,hamster,rabbit and rhesus monkey.The cercariae applied for infection were shed by snails collected from 5 different isolates,i.e.,Anhui,Hubei,Sichuan,Yunnan and Guangxi in the mainland of China.Sera from the same sort of animals were separately pooled according to the different sources of infection mentioned above.Antibody reactivities were evaluated by COPT,LAT and ELISA.In COPT each serum sample was tested with ova from homologous and heterologous isolates,whereas soluble egg antigen merely prepared from Anhui isolate was used in LAT and ELISA.The level of antibodies detected by homologous or heterologous worm isolate antigens was compared.The results suggested that the positive antibody defection rate might not be influenced by antigens prepared from S.japonicum eggs of different isolates in the mainland of China.