中国寄生虫学与寄生虫病杂志 ›› 1991, Vol. 9 ›› Issue (3): 209-212.

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三种蚤酯酶同工酶的比较分析

曹丽萍,何麟   

  1. 广州军区广州总医院血液室; 第一军医大学寄生虫学教研室
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1991-08-31 发布日期:2017-01-09

STUDIES ON ESTERASE ISOENZYME OF THREE SPECIES OF FLEA

  • Received:2017-01-09 Revised:2017-01-09 Online:1991-08-31 Published:2017-01-09

摘要: 应用聚丙烯酰胺凝胶垂直平板电泳(PAGE)对印鼠客蚤、猫栉首蚤指名亚种和秃病蚤蒙冀亚种进行了酯酶同工酶的比较分析,结果发现蚤类酶谱存在差异,3种蚤约有20—22条迁移率不同的酶带。印鼠客蚤约显示14条带,主带5-6条;猫蚤显示9条带,主带4条:秃病蚤亦显示9条带;主带4—5条带。3种蚤间有共同的主带和次级酶带,亦有各自特异的标志酶带。吸血前后的雌、雄成虫酶谱基本一致,而成虫与幼虫间略有差异。所以酯酶同工酶酶谱的差异和特异性,是蚤类分类鉴定的一个分子遗传学指标。

关键词: 蚤, 酯酶, 同工酶, 遗传学

Abstract: The patterns of esterase (Est) isoenzyme of 3 flea species were studied by vertical slab polyacrylamide gel e.ectrphoresis (PAGE). The samples prepared from the third larval ins-tar, female and male (the unsucking and sucked one) were run in the same gel slab. The result was as follows: Xenopsylla cheopis showed 14 bands, with 5-6 major bands, Cte-nocephalides felis felis showed 9 bands with 4-5 major bands and Nosopsyllus laeviceps kuzenkovi showed 9 bands with 4-5 major bands. These three species have their common bands as well as respective specific bands. The difference in Est isoenzymogram among families was greater than among genera, displaying a species-specific feature (Figs. 1-4).