中国寄生虫学与寄生虫病杂志 ›› 1991, Vol. 9 ›› Issue (3): 169-172.

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单克隆抗体夹心斑点免疫金银染色法诊断恶性疟的初步研究

周增桓,李海燕,李英杰,巢穗,欧阳明辉   

  1. 第一军医大学疟疾免疫研究室
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1991-08-31 发布日期:2017-01-09

PRELIMINARY STUDIES ON THE DIAGNOSIS OF PLASMODIVM FALCIPARVM MALARIA BY MONOCLONAL ANTIBODY SANDWICH DOT-IMMUNOGOLD SILVER STAINING ASSAY

  • Received:2017-01-09 Revised:2017-01-09 Online:1991-08-31 Published:2017-01-09

摘要: 用单克隆抗体(McAb)夹心斑点免疫金银染色法(Dot-IGSSA),对采自海南省的30价原虫血症范围为0.015-0.58%的恶性疟病人血和30份正常人血进行了检测。当用McAb 11G_5、13A_2、13A_1分别与金标记McAb 14D_9夹心时,其阳性检出率分别为96.7%、93.3%和93.3%,假阳性反应约为3.3%,最低可检测出约0.0001%的疟原虫血症。其中11G_5、13A_1分别与金标记14D_9夹心时,对体外培养的云南和安徽的恶性疟红内期抗原呈阳性反应,但其McAb与诺氏疟原虫和伯氏疟原虫均无交叉反应。McAb 13A_1与14D_9夹心时,对食蟹猴疟原虫和间日疟病人

关键词: 恶性疟原虫, 疟疾诊断, 单克隆抗体, 斑点免疫金银染色法

Abstract: In this report, the blood samples from 30 falciparum malaria patients with parasftemia 0.015-0.58% and the blood samples from 30 healthy persons were examined by monoclonal antibody (McAb) sandwich dot-immunogold silver staining assay (Dot-IGSSA). When the McAb 11G5, 13A2 and 13A1 were used for sandwith Dot-IGSSA with McAb 14D9 labeled with colloidal gold respectively, the 0.0001% of parasitemia could be detected and the McAb 11G5, 13A1 and 14D9 labeled with colloidal gold could also be used to detect the an-tigens of asexual blood stages of Yunnan and Anhui isolates of Plasmodium falciparum cultured in vitro. These McAbs did not cross-react with the antigens of Plos .Mum know-lesi and Plasmodium berghei, however, the McAbs 13A1 and 14D9 weakly cross-reacted with the antigens of Plasmodium cynomolgi and the antigens in the infected blood samples from patients with vivax malaria (Fig. 1).