中国寄生虫学与寄生虫病杂志 ›› 1986, Vol. 4 ›› Issue (3): 215-217.

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人蛲虫卵卵壳的光镜及扫描电镜观察

戴世忠,刘振兴,李贵生,吴祖明   

  1. 湛江医学院
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1986-08-31 发布日期:2017-01-12

LIGHT AND SCANNING ELECTRON MICROSCOPIC OBSERVATION OF THE EGG-SHELL OF ENTEROBIVS VERMICULARIS

  • Received:2017-01-12 Revised:2017-01-12 Online:1986-08-31 Published:2017-01-12

摘要: 用蜡纸垫圈玻片法取自然产出的人蛲虫卵混入1:3蛋白液中制成玻片标本进行光镜观察,结果表明人蛲虫卵的立体外形为椭球体,在卵壳较凸出一侧的外表有一对纵行并列的背脊,并经石蜡切片和扫描电镜证实.在扫描电镜下可见卵壳表面布满间距不等的微孔,在卵小端两背脊末稍处有一粗糙小区,是幼虫的孵出口。分析了同一虫卵在镜下呈现不对称形状多于对称形状的原因。

关键词: 人蛲虫卵, 扫描电镜观察, 不对称, 对称形, 鼠管状线虫, 卵壳表面, 长椭球体, 长椭圆形, 玻片标本, 医学寄生虫学

Abstract: Spontaneously deposited eggs of E. vermicularis were suspended in 1:3 egg white mucilage and mounted on slides previously fixed with wax paper frame to avoid pressure of the coverslide and to give space to eggs deposited in any position. The variation of the contour of the egg shell at various planes was observed and a stereoscopic configuration of egg shell obtained.The egg-shell of E. vermicularis is asymmetrically ellipsoid. A pair of longitudinal ridges run parallelly over the convex or dorsal side of the shell. Scanning electron microscopy showed the presence of numerous minute pores distributed unequally over the shell surface and a foveola with an uneven base situated on the dorsal ridges. It is considered to be the hatching pore of the egg. The reason why eggs can present both asymmetric and symmetric contours is discussed.