中国寄生虫学与寄生虫病杂志 ›› 1984, Vol. 2 ›› Issue (3): 170-172.

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贾第虫的培养及其分裂繁殖情况的观察

张月清,王正仪,卢思奇,温艳,桂淑环,赵森林   

  1. 北京热带医学研究所; 北京热带医学研究所; 北京热带医学研究所; 北京热带医学研究所; 北京热带医学研究所; 北京临床医学研究所
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1984-08-31 发布日期:2017-01-12

CULTIVATION OF GIARDIA LAMBLIA AND CERTAIN OBSERVATIONS ON ITS BINARY FISSION PROCESS

  • Received:2017-01-12 Revised:2017-01-12 Online:1984-08-31 Published:2017-01-12

摘要: 用于单栖培养的贾第虫株系从患者粪便中分离包囊,用蔗糖梯度离心提纯后感染家兔,再从感染兔肠粘膜取滋养体,接种在同时种有Saccharomyces cerevisiae悬液的培养基中,37℃培养24小时,生长最旺,此后虫体随酵母的增多而减少。用于纯培养的虫株及TPS-1培养基由美国疾病控制中心提供。在5个月内传代32次。用扫描电镜和光镜观察了虫体分裂繁殖的某些形态特点。

关键词: 贾第虫, 分裂繁殖, 纯培养, 热带医学, 扫描电镜, 分裂沟, 滋养体, 酵母, 细胞周期, 冰冻保存

Abstract: Certain characteristics concerning the growth and multiplication of Giardia lamblia were observed in the course of monoxenic and axenic cultivation of the parasite. The organism used for monoxenic culture originated from a local giardiasis patient. Cysts from patient fecal specimen were isolated by sucrose gradient centrifugation and then used to infect rabbits. Trophozoites recovered from intestinal mucosa of the infected animal were cultivated at 37℃ together with a small amount of Saccharomyces cerevisiae. They grew luxuriantly and formed a monolayer upon the wall of the culture tube at about 24 hours. Thereafter, yeast cells gradually overgrew the parasite whose number becomes less and less, until very few remains at the fifth day.Strain of G. lamblia and TPS-1 medium used in the axenic culture was supplied by Dr. Visvesvara (CDC, Atlanta). Certain characteristics of the multiplication of the parasite, particularly those concerning the binary fission process, were observed under scanning electron microscope and optic microscope during the course of cultivation which lasted 5 months. The morphological changes at various stages of binary fission of the organism were described.