关键词: 日本血吸虫抗原, ELISA法检测, 抗原抗体复合物, 抗原活性, 抗体活性, 酶催化反应, 慢性血吸虫病, 酶标记抗体, 反应速率, 同时测定

Abstract: K-ELISA technique was employed to compare the potencies of various schistosome antigens and their corresponding antibodies.Pooled serum was prepared from 3 documented cases of chronic schistosomiasis and served as standard serum. The antigenic potencies of JAMA, SEA, JEU, JEC1, JEC2, and JEC3 were found to be 2.0, 8.0, 4.0, 4.0, 5.0 and 10.0 (AA460/min × 10-2) respectively, JEC3 being apparently the most potent one.The mean serum antibody potencies (AA 460/min× 10-2) of schistosomiasis patients before and after pyquiton treatment were 8.110.3 and 3.010.2 (n = 12) for anti-JAMA; 12.8±0.3 and 9.0 ±2.0 (n = 12) for anti-SEA; 6.2±0.6 and 3.810.4 (n = 30) for anti-JEU; 6.8±0.6 and 4.8±0.4 (n = 30) for anti-JEC2 11.7±0.8 and 2.1±0.4 (n = 30) for anti-JEC3. Those for controls (n = 16) were 0.7±0.7, 1.5±0.5, 2.8±0.6, 1.8±0.2 and 2.0±0.4, respectively.It is suggested that JEC3 might be served as a satisfactory diagnostic antigen and K-ELISA might be a useful laboratory method in the study of schistosome antigens and antibodies.