中国寄生虫学与寄生虫病杂志 ›› 1984, Vol. 2 ›› Issue (2): 111-113.

• • 上一篇    下一篇

中华按蚊体内马来丝虫幼虫的放射性标记

朱素贞,钟永良,刘美涛,张选明,饶忠秀,郑德福,蔡学林,郭大智,苏全君,廖兴玉,宋雪华   

  1. 四川省寄生虫病防治研究所; 四川省寄生虫病防治研究所; 四川省寄生虫病防治研究所; 四川省寄生虫病防治研究所; 四川省寄生虫病防治研究所; 四川省寄生虫病防治研究所; 四川农学院原子能农业应用研究室; 四川农学院原子能农业应用研究室; 四川农学院原子能农业应用研究室; 四川农学院原子能农业应用研究室; 四川农学院原子能农业应用研究室
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1984-05-31 发布日期:2017-01-12

RADIOISOTOPIC LABELLING OF THIRD-STAGE LARVAE OF BRVGIA MALAYI IN ANOPHELES SINENSIS

  • Received:2017-01-12 Revised:2017-01-12 Online:1984-05-31 Published:2017-01-12

摘要: 通过膜饲吸血法,使中华按蚊同时吸入马来丝虫微丝蚴和标记物(~(35)S-蛋氨酸、~3H-尿嘧啶核苷和~(32)P-磷酸二氢钠)。待微丝蚴发育至第三期幼虫后分离并测定其放射性。结果表明幼虫都成功地获得了标记,但幼虫获得标记物的量并不相同,成蚊和幼虫获得的~(35)S-蛋氨酸标记量均较~(32)P-磷酸二氢钠为高。提示成蚊和幼虫对不同营养物具有选择性。本文为研究马来丝虫幼虫的生物学特性提供了一个接近自然条件的体内标记方法。

关键词: 马来丝虫, 中华按蚊, 放射性标记物, 感染期幼虫, 生物学特性, 磷酸二氢钠, 蛋氨酸, 放射性物质, 防治研究, 寄生虫病

Abstract: The incorporation ire vivo of 35S-methionine, 3H-uridine and 32P-NaH2PO4 by the third-stage infective larvae in A, sinensis was investigated respectively by means of membranous feeding. The quantity of the three radioisotopes in these larvae were not equal. For example when 35S-methionine and 32P-NaH2PO4 were given to mosquitoes in almost equal quantity, the radioactivity was higher for the former than the latter in both the mosquitoes and larvae. The present study has provided an in vivo method for studying the biology of B. malayi larvae under the natural condition.