中国寄生虫学与寄生虫病杂志 ›› 1984, Vol. 2 ›› Issue (1): 21-24.

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日本血吸虫表皮膜蛋白的分离与免疫活性鉴定

李筠,赵惠芬,叶雪玲,沈菊英,杜端杰   

  1. 中国科学院上海生物化学研究所; 上海市血吸虫病防治研究所; 中国科学院上海生物化学研究所; 中国科学院上海生物化学研究所; 中国科学院上海生物化学研究所
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1984-02-28 发布日期:2017-01-12

ISOLATION AND 1MMUNOACTIVE CHARACTERIZATION OFTEGUMENTAL MEMBRANE PROTEINS FROM ADULTSCHISTOSOMA JAPONICVM

  • Received:2017-01-12 Revised:2017-01-12 Online:1984-02-28 Published:2017-01-12

摘要: 日本血吸虫表皮膜粗制剂,用阴离子去垢剂十二烷基磺酸钠提取,除去水溶性杂蛋白及其他疏松结合于膜上的蛋白,离心后分离得到紧密结合于脂双层膜中的膜蛋白。SDS-聚丙烯酰胺凝胶电泳显示主要为5个蛋白质条带,其分子量分别为:Ⅰ64,000;Ⅱ58,000;Ⅲ39,000;Ⅳ27,000;Ⅴ18,7000。其中Ⅰ、Ⅲ、Ⅳ3个蛋白含有糖。应用固定化葡萄球菌A蛋白吸附~(125)Ⅰ标记的抗原-抗体复合物及放射自显影鉴定,其中分子量为39,000和27,000的膜蛋白具有免疫活性。

关键词: 日本血吸虫, 表皮膜蛋白, 免疫活性, 曼氏血吸虫, 葡萄球菌A蛋白, 阴离子去垢剂, 脂双层膜, 抗体复合物, 抗血清, 表观分子量

Abstract: The integral tegumental membrane proteins have been obtained from the crude tegument membrane preparation by the extraction and removal of the water soluble proteins and other peripheral protein components with sodium lauryl sulphate. Five' membrane proteins were identified by SDS-PAGE electrophoresis with the following molecular weight: Ⅰ. 64,000; Ⅱ. 58,000; Ⅲ. 39,000; Ⅳ. 27,000 and Ⅴ. 18,700. The membrane proteins of Ⅰ, Ⅲ and Ⅳ are glycoproteins. Among these membrane proteins two components can be recognized by specific antibodies, based upon the isolation of 125I labelled immune complexes with Staphylococcus Protein A, SDS-PAGE electrophoresis and autoradiography.