关键词: 日本血吸虫, 乳清酸, 酶活力, 磷酸核糖, 碳酸氢钠, 转移酶, 合成代谢, 合成途径, 硝硫氰胺, 酸合成酶

Abstract: Experiments on the pyrimidine metabolism in Schistosoma japonicum have demonstrated that labelled bicarbonate, orotic acid and uracil are all incorporated into the nucleic acids of worms. This indicates that carbamyl phosphate synthetase II-aspartate carbamyl transferase-dihydroorotase complex, dihydroorotate dehydrogenase as well as orotate phosphoribosyl-transferase-orotidylate decarboxylase complex do exist.S. japonicum not only possesses all the enzymes of the pyrimidine de novo pathway but also the capacity of salvaging the preformed pyrimidines. It is evident that host blood may supply sufficient amount of bicarbonate to meet the requirements of de novo synthesis, but it is questionable whether blood contains enough quantity of pyrimidine bases and nucleosides for the salvage pathway.The effect of schistosomicides (niridazole and amoscanate) on the pyrimidine de novo pathway in vivo has also been studied. It is suggested that the orotate phosphoribosyl-transferase-orotidylate decarboxylase complex is a vulnerable point which might be attacked by chemotherapeutic agents. Since the structure of niridazole is similar to that of orotic acid, analogues of orotic acid may be of selective significance.