关键词: 日本血吸虫病, 环卵沉淀试验, 血吸虫病防治, 环沉率, 联苯胺, 血清流行病学调查, 阳性率, ELISA, 反应物, 环卵沉淀反应

Abstract: A modified ELCOPT technique using heat-treated, ultrasonicated dry egg antigen was reported. The relative sensitivity of this method in 327 parasitologically confirmed cases was compared with those of COPT, ELISA and IHA. The positive rate of ELCOPT was 96.0% (314/327) as against 94.2% (308/327) of COPT, the difference being statistically insignificant (P0.05); the average percentage of ova with precipitates in the former was 27.9% as against 15.7% in the latter, the difference being highly significant (P0.01); the positive rates of ELISA and IHA were 89.6% (293/327) (P0.01)and 82.1% (87/106) (P0.05) respectively. Sera from 43 healthy persons all showed negative with ELCOPT; when sera of 87 children tentatively diagnosed as hepatitis below 15 years of age were assayed, only 3 cases (3.5%) produced positively colored precipitates, the percentage of ova with precipitates was 1-2%. The microplate method of ELCOPT presented here using heat-treated, ultrasonicated, dry egg antigen and the appropriate substrate, 0.4% benzidine (4,4-diamino-biphenyl benzidine) might be a useful tool applicable in large scale seroepidemiological surveys.