中国寄生虫学与寄生虫病杂志 ›› 2019, Vol. 37 ›› Issue (2): 232-234.doi: 10.12140/j.issn.1000-7423.2019.02.021

• 研究简报 • 上一篇    下一篇

荧光定量PCR在疟疾实验室诊断中的应用

李素华1(), 李静2, 高丽君1, 张雅兰1, 周瑞敏1, 钱丹1, 杨成运1, 刘颖1, 赵玉玲1,*(), 张红卫1   

  1. 1 河南省疾病预防控制中心,郑州 450016
    2 河南国际旅行卫生保健中心,郑州 450046
  • 收稿日期:2018-07-16 出版日期:2019-04-30 发布日期:2019-05-13
  • 通讯作者: 赵玉玲
  • 作者简介:

    作者简介:李素华(1988-),女,硕士,主管技师,从事寄生虫病研究。E-mail:lsh0427@163.com

  • 基金资助:
    河南省医学科技攻关计划项目(No. 201702274, No. 162102310066);河南省科技攻关项目(No. 162102310035)

Application of fluorescence quantitative PCR in laboratory diagnosis of malaria

Su-hua LI1(), Jing LI2, Li-jun GAO1, Ya-lan ZHANG1, Rui-min ZHOU1, Dan QIAN1, Cheng-yun YANG1, Ying LIU1, Yu-ling ZHAO1,*(), Hong-wei ZHANG1   

  1. 1 Henan Center for Disease Control and Prevention, Zhengzhou 450016, China
    2 Henan International Travel Healthcare Center, Zhengzhou 450046, China
  • Received:2018-07-16 Online:2019-04-30 Published:2019-05-13
  • Contact: Yu-ling ZHAO
  • Supported by:
    Supported by the Project of Medical Science and Technology of Henan Province (No. 201702274, No. 162102310066);Science and Technology of Henan Province (No. 162102310035)

摘要:

分别采用镜检、巢式PCR和荧光定量PCR等3种检测方法对收集到的79份疟疾血样进行检测,并对检测结果进行比较分析。结合患者临床症状和3种检测结果,79份血样最终确诊74份为疟原虫阳性,阳性率为93.7%(74/79),其中镜检阳性检出率为82.3%(65/79),巢式PCR阳性检出率为82.3%(65/79),荧光定量PCR阳性检出率为93.7%(74/79),荧光定量PCR阳性检出率高于其他两种方法(P < 0.05)。镜检和巢式PCR的一致率为78.4%(58/74),镜检和荧光定量PCR的一致率为63.5%(47/74),巢式PCR和荧光定量PCR的一致率为83.8%(62/74)。相比镜检和巢式PCR,荧光定量PCR敏感性和检出率更高,且用时更短。

关键词: 疟疾, 镜检, 巢式PCR, 荧光定量PCR

Abstract:

Seventy-nine malaria samples were examined by microscopy, nested PCR and fluorescence quantitative PCR, and the detection rate was analyzed among the three methods. After combining clinical symptoms of the patients and results of the three methods, 74 of the 79 samples were confirmed to be malaria infection, with a positive rate of 93.7%(74/79). Specifically, the detection rate of microscopy, nested PCR and fluorescence quantitative PCR were 82.3%(65/79), 82.3%65/79) and 93.7%(74/79), respectively. The concordance rate between microscopy and nested PCR was 78.4%(58/74), and that between microscopy and fluorescence quantitative PCR was 63.5%(47/74), between nested PCR and fluorescence quantitative PCR was 83.8%(62/74). In addition, the detection rate of fluorescence quantitative PCR was significantly higher than that of the nested PCR(P < 0.05). Compared with microscopy and nested PCR, the fluorescence quantitative PCR has higher sensitivity and positive detection rate, and is less time-consuming.

Key words: Malaria, Microscopy, Nested PCR, Fluorescence quantitative PCR

中图分类号: